Previous efforts to visualize Notch receptor expression have focused on lin-12 expression in the soma and glp-1 expression in the germline and early embryo. LIN-12 has been visualized successfully using LIN-12::GFP fusion proteins introduced via traditional, multi-copy transgenic techniques (e.g. Levitan and Greenwald, 1998; Sarov et al., 2012). Such techniques have been difficult for visualizing glp-1 expression in the germline, until recently (Cinquin et al., 2015; Gutnik et al., 2018), due to germline silencing of multi-copy transgenes (Kelly et al., 1997; Merritt and Seydoux, 2010). Visualizing GLP-1expression in the germline has therefore relied on antibody staining, primarily using an antibody raised against the GLP-1 extra-cellular domain (Crittenden et al., 1994). This antibody reveals easily detectable GLP-1 protein at the plasma membrane in the distal germline and early embryos (Crittenden et al., 1994; Evans et al., 1994), but it does not allow visualization of the GLP-1 nuclear intracellular domain (NICD), which moves into the nucleus upon receptor activation. This antibody is therefore not useful for identifying cells that have directly received active GLP-1 signaling. To overcome this limitation, and to broaden the toolkit for visualizing glp-1 expression, we created five strains expressing tagged glp-1 alleles, including transgenes and CRISPR tags at the endogenous locus. We report here the expression patterns of the tagged glp-1 alleles, focusing on the adult gonad.
CCG Toolkit v1.1
2ff7e9595c
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